A fundamental challenge in modern science is to understand how the brain functions in health and disease. We are interested in elucidating the mechanisms leading to neurological diseases and ageing, in particular stroke and dementia. To this end, we develop and implement 3D imaging technologies to be able to generate the highest resolution views of intact rodent organs and bodies. We developed DISCO line of organic solvent tissues clearing methods, which have been a great utility for scientists from diverse bio-medical research fields. Overall, this approach provides us with a holistic view of inter connected biological systems to comprehend the disease induced mechanisms in an unbiased way.